Missions
In the frame of an Euramet-funded european project (ProMET) involving National Institutes of Metrology and international structural mass spectrometry groups, the candidate will develop H/D exchange MS (HDX-MS) and Ion Mobility-MS (IM-MS) for the characterisation and quantification of protein conformational states and interactions. The goal is to assess the impact of small primary sequence changes on the higher order structure of reference protein materials.
Activities
Activities
- Design, perform and interpret HDX-MS and IM-MS on a series of reference material samples.
- Perform crosslinking mass spectrometry (XL-MS) analyses as orthogonal technique
- Bioinformatics interpretation of data.
- Monitor project(s)
- participate and present results in consortium meetings.
- Write reports.
- Present, disseminate and promote achievements.
- Ensure traceability of analyses as part of LSMBO's quality approach (ISO9001 environment).
Skills
General knowledge
- Advanced knowledge in bioanalytical chemistry, mass spectrometry-based protein analysis.
Operational know-how
- In-depth knowledge in intact protein analysis using mass spectrometry
- Knowledge in nanoLC-MS/MS proteomic analysis;
- knowledge in HDX-MS, IM-MS and XL-MS (chemical crosslinking MS).
- Master liquid chromatography (reverse-phase chromatography) and tandem mass spectrometry techniques;
- Master specific tools and software for reprocessing mass spectrometry data (Dynamix, Proteome Discoverer, MaxQuant, etc.).
- Manage work in an ISO9001-certified environment;
- Communicate with internal and external contacts;
- Work as part of a team in a multidisciplinary environment;
- Communicate in english (written and spoken) at level B2 according to the Common European Framework of Reference for Languages so as to be able to make regular reports and present results to English-speaking colleagues.
More information
- Application deadline: 30 October 2024 23:59:00 Paris time
- Application Web page: https://emploi.cnrs.fr/Offres/CDD/UMR7178-REGSOM-206/Default.aspx?lang=EN
